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superscript ii rt-pcr kit  (Thermo Fisher)


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    Thermo Fisher superscript ii rt-pcr kit
    Superscript Ii Rt Pcr Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+ii+rt-pcr+kit/pmc11782520-343-18-22
    Average 90 stars, based on 1 article reviews
    superscript ii rt-pcr kit - by Bioz Stars, 2026-09
    90/100 stars

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    Reverse Transcription:


    Article Title: EZH2 serves as a viable therapeutic target for myeloma-induced osteolytic bone destruction.
    Article Snippet: Aquantity of 1 μgof the total RNAwas subsequently utilized for reverse transcription (RT) employing the SuperScript II RT-PCR kit (Invitrogen).

    Article Title: LINC00467 Is Upregulated by DNA Copy Number Amplification and Hypomethylation and Shows ceRNA Potential in Lung Adenocarcinoma.
    Article Snippet: After 48 h of 5-azacytidine treatment, the total RNA in the A549 cells was isolated using Trizol reagent (Thermo Fisher Scientific). cDNA synthesis was conducted using a SuperScript II RT-PCR kit (Life Technologies), while quantitative PCR was performed using the SYBR Green Master Mix (Life Technologies) with the QuantStudio 3 Real-Time PCR System (Life Technologies) according to the manufacturer’s instructions.

    Article Title: Mks6 mutations reveal tissue- and cell type-specific roles for the cilia transition zone
    Article Snippet: Quantitative RT-PCR RNA was isolated from Mks6 wild type ( WT ) and Mks6 GT MEFs with Trizol reagent, according to the manufacturer’s protocol (15596-026; Thermo Fisher Scientific, Waltham, MA, USA). cDNA was generated using Superscript II RT-PCR kit (18064; Thermo Fisher Scientific).

    Article Title: EZH2 serves as a viable therapeutic target for myeloma-induced osteolytic bone destruction
    Article Snippet: A quantity of 1 μg of the total RNA was subsequently utilized for reverse transcription (RT) employing the SuperScript II RT-PCR kit (Invitrogen).

    Article Title: TGFBR1 Variants Can Associate with Non-Syndromic Congenital Heart Disease without Aortopathy
    Article Snippet: First-strand cDNA was synthesized from this isolated total RNA (1 μg for both the + RT and -RT reaction) using oligo(dT) primer and the Superscript II RT-PCR kit (Invitrogen, Waltham, MA, USA) in a 25 μL reaction according to the manufacturer’s procedure.

    Article Title: LINC00467 Is Upregulated by DNA Copy Number Amplification and Hypomethylation and Shows ceRNA Potential in Lung Adenocarcinoma
    Article Snippet: After 48 h of 5-azacytidine treatment, the total RNA in the A549 cells was isolated using Trizol reagent (Thermo Fisher Scientific). cDNA synthesis was conducted using a SuperScript II RT-PCR kit (Life Technologies), while quantitative PCR was performed using the SYBR Green Master Mix (Life Technologies) with the QuantStudio 3 Real-Time PCR System (Life Technologies) according to the manufacturer’s instructions.

    Article Title: Brief inhalation of sevoflurane can reduce glial scar formation after hypoxic-ischemic brain injury in neonatal rats
    Article Snippet: Complementary DNA was synthesized using an advanced Superscript II RT-PCR kit (Invitrogen).

    Reverse Transcription Polymerase Chain Reaction:


    Article Title: EZH2 serves as a viable therapeutic target for myeloma-induced osteolytic bone destruction.
    Article Snippet: Aquantity of 1 μgof the total RNAwas subsequently utilized for reverse transcription (RT) employing the SuperScript II RT-PCR kit (Invitrogen).

    Article Title: LINC00467 Is Upregulated by DNA Copy Number Amplification and Hypomethylation and Shows ceRNA Potential in Lung Adenocarcinoma.
    Article Snippet: After 48 h of 5-azacytidine treatment, the total RNA in the A549 cells was isolated using Trizol reagent (Thermo Fisher Scientific). cDNA synthesis was conducted using a SuperScript II RT-PCR kit (Life Technologies), while quantitative PCR was performed using the SYBR Green Master Mix (Life Technologies) with the QuantStudio 3 Real-Time PCR System (Life Technologies) according to the manufacturer’s instructions.

    Article Title: Mks6 mutations reveal tissue- and cell type-specific roles for the cilia transition zone
    Article Snippet: Quantitative RT-PCR RNA was isolated from Mks6 wild type ( WT ) and Mks6 GT MEFs with Trizol reagent, according to the manufacturer’s protocol (15596-026; Thermo Fisher Scientific, Waltham, MA, USA). cDNA was generated using Superscript II RT-PCR kit (18064; Thermo Fisher Scientific).

    Article Title: EZH2 serves as a viable therapeutic target for myeloma-induced osteolytic bone destruction
    Article Snippet: A quantity of 1 μg of the total RNA was subsequently utilized for reverse transcription (RT) employing the SuperScript II RT-PCR kit (Invitrogen).

    Article Title: TGFBR1 Variants Can Associate with Non-Syndromic Congenital Heart Disease without Aortopathy
    Article Snippet: First-strand cDNA was synthesized from this isolated total RNA (1 μg for both the + RT and -RT reaction) using oligo(dT) primer and the Superscript II RT-PCR kit (Invitrogen, Waltham, MA, USA) in a 25 μL reaction according to the manufacturer’s procedure.

    Article Title: LINC00467 Is Upregulated by DNA Copy Number Amplification and Hypomethylation and Shows ceRNA Potential in Lung Adenocarcinoma
    Article Snippet: After 48 h of 5-azacytidine treatment, the total RNA in the A549 cells was isolated using Trizol reagent (Thermo Fisher Scientific). cDNA synthesis was conducted using a SuperScript II RT-PCR kit (Life Technologies), while quantitative PCR was performed using the SYBR Green Master Mix (Life Technologies) with the QuantStudio 3 Real-Time PCR System (Life Technologies) according to the manufacturer’s instructions.

    Article Title: Brief inhalation of sevoflurane can reduce glial scar formation after hypoxic-ischemic brain injury in neonatal rats
    Article Snippet: Complementary DNA was synthesized using an advanced Superscript II RT-PCR kit (Invitrogen).

    Synthesized:


    Article Title: EZH2 serves as a viable therapeutic target for myeloma-induced osteolytic bone destruction.
    Article Snippet: Aquantity of 1 μgof the total RNAwas subsequently utilized for reverse transcription (RT) employing the SuperScript II RT-PCR kit (Invitrogen).

    Article Title: LINC00467 Is Upregulated by DNA Copy Number Amplification and Hypomethylation and Shows ceRNA Potential in Lung Adenocarcinoma.
    Article Snippet: After 48 h of 5-azacytidine treatment, the total RNA in the A549 cells was isolated using Trizol reagent (Thermo Fisher Scientific). cDNA synthesis was conducted using a SuperScript II RT-PCR kit (Life Technologies), while quantitative PCR was performed using the SYBR Green Master Mix (Life Technologies) with the QuantStudio 3 Real-Time PCR System (Life Technologies) according to the manufacturer’s instructions.

    Article Title: Mks6 mutations reveal tissue- and cell type-specific roles for the cilia transition zone
    Article Snippet: Quantitative RT-PCR RNA was isolated from Mks6 wild type ( WT ) and Mks6 GT MEFs with Trizol reagent, according to the manufacturer’s protocol (15596-026; Thermo Fisher Scientific, Waltham, MA, USA). cDNA was generated using Superscript II RT-PCR kit (18064; Thermo Fisher Scientific).

    Article Title: EZH2 serves as a viable therapeutic target for myeloma-induced osteolytic bone destruction
    Article Snippet: A quantity of 1 μg of the total RNA was subsequently utilized for reverse transcription (RT) employing the SuperScript II RT-PCR kit (Invitrogen).

    Article Title: TGFBR1 Variants Can Associate with Non-Syndromic Congenital Heart Disease without Aortopathy
    Article Snippet: First-strand cDNA was synthesized from this isolated total RNA (1 μg for both the + RT and -RT reaction) using oligo(dT) primer and the Superscript II RT-PCR kit (Invitrogen, Waltham, MA, USA) in a 25 μL reaction according to the manufacturer’s procedure.

    Article Title: LINC00467 Is Upregulated by DNA Copy Number Amplification and Hypomethylation and Shows ceRNA Potential in Lung Adenocarcinoma
    Article Snippet: After 48 h of 5-azacytidine treatment, the total RNA in the A549 cells was isolated using Trizol reagent (Thermo Fisher Scientific). cDNA synthesis was conducted using a SuperScript II RT-PCR kit (Life Technologies), while quantitative PCR was performed using the SYBR Green Master Mix (Life Technologies) with the QuantStudio 3 Real-Time PCR System (Life Technologies) according to the manufacturer’s instructions.

    Article Title: Brief inhalation of sevoflurane can reduce glial scar formation after hypoxic-ischemic brain injury in neonatal rats
    Article Snippet: Complementary DNA was synthesized using an advanced Superscript II RT-PCR kit (Invitrogen).

    Isolation:


    Article Title: EZH2 serves as a viable therapeutic target for myeloma-induced osteolytic bone destruction.
    Article Snippet: Aquantity of 1 μgof the total RNAwas subsequently utilized for reverse transcription (RT) employing the SuperScript II RT-PCR kit (Invitrogen).

    Article Title: LINC00467 Is Upregulated by DNA Copy Number Amplification and Hypomethylation and Shows ceRNA Potential in Lung Adenocarcinoma.
    Article Snippet: After 48 h of 5-azacytidine treatment, the total RNA in the A549 cells was isolated using Trizol reagent (Thermo Fisher Scientific). cDNA synthesis was conducted using a SuperScript II RT-PCR kit (Life Technologies), while quantitative PCR was performed using the SYBR Green Master Mix (Life Technologies) with the QuantStudio 3 Real-Time PCR System (Life Technologies) according to the manufacturer’s instructions.

    Article Title: Mks6 mutations reveal tissue- and cell type-specific roles for the cilia transition zone
    Article Snippet: Quantitative RT-PCR RNA was isolated from Mks6 wild type ( WT ) and Mks6 GT MEFs with Trizol reagent, according to the manufacturer’s protocol (15596-026; Thermo Fisher Scientific, Waltham, MA, USA). cDNA was generated using Superscript II RT-PCR kit (18064; Thermo Fisher Scientific).

    Article Title: EZH2 serves as a viable therapeutic target for myeloma-induced osteolytic bone destruction
    Article Snippet: A quantity of 1 μg of the total RNA was subsequently utilized for reverse transcription (RT) employing the SuperScript II RT-PCR kit (Invitrogen).

    Article Title: TGFBR1 Variants Can Associate with Non-Syndromic Congenital Heart Disease without Aortopathy
    Article Snippet: First-strand cDNA was synthesized from this isolated total RNA (1 μg for both the + RT and -RT reaction) using oligo(dT) primer and the Superscript II RT-PCR kit (Invitrogen, Waltham, MA, USA) in a 25 μL reaction according to the manufacturer’s procedure.

    Article Title: LINC00467 Is Upregulated by DNA Copy Number Amplification and Hypomethylation and Shows ceRNA Potential in Lung Adenocarcinoma
    Article Snippet: After 48 h of 5-azacytidine treatment, the total RNA in the A549 cells was isolated using Trizol reagent (Thermo Fisher Scientific). cDNA synthesis was conducted using a SuperScript II RT-PCR kit (Life Technologies), while quantitative PCR was performed using the SYBR Green Master Mix (Life Technologies) with the QuantStudio 3 Real-Time PCR System (Life Technologies) according to the manufacturer’s instructions.

    Article Title: Brief inhalation of sevoflurane can reduce glial scar formation after hypoxic-ischemic brain injury in neonatal rats
    Article Snippet: Complementary DNA was synthesized using an advanced Superscript II RT-PCR kit (Invitrogen).

    cDNA Synthesis:


    Article Title: EZH2 serves as a viable therapeutic target for myeloma-induced osteolytic bone destruction.
    Article Snippet: Aquantity of 1 μgof the total RNAwas subsequently utilized for reverse transcription (RT) employing the SuperScript II RT-PCR kit (Invitrogen).

    Article Title: LINC00467 Is Upregulated by DNA Copy Number Amplification and Hypomethylation and Shows ceRNA Potential in Lung Adenocarcinoma.
    Article Snippet: After 48 h of 5-azacytidine treatment, the total RNA in the A549 cells was isolated using Trizol reagent (Thermo Fisher Scientific). cDNA synthesis was conducted using a SuperScript II RT-PCR kit (Life Technologies), while quantitative PCR was performed using the SYBR Green Master Mix (Life Technologies) with the QuantStudio 3 Real-Time PCR System (Life Technologies) according to the manufacturer’s instructions.

    Article Title: Mks6 mutations reveal tissue- and cell type-specific roles for the cilia transition zone
    Article Snippet: Quantitative RT-PCR RNA was isolated from Mks6 wild type ( WT ) and Mks6 GT MEFs with Trizol reagent, according to the manufacturer’s protocol (15596-026; Thermo Fisher Scientific, Waltham, MA, USA). cDNA was generated using Superscript II RT-PCR kit (18064; Thermo Fisher Scientific).

    Article Title: EZH2 serves as a viable therapeutic target for myeloma-induced osteolytic bone destruction
    Article Snippet: A quantity of 1 μg of the total RNA was subsequently utilized for reverse transcription (RT) employing the SuperScript II RT-PCR kit (Invitrogen).

    Article Title: TGFBR1 Variants Can Associate with Non-Syndromic Congenital Heart Disease without Aortopathy
    Article Snippet: First-strand cDNA was synthesized from this isolated total RNA (1 μg for both the + RT and -RT reaction) using oligo(dT) primer and the Superscript II RT-PCR kit (Invitrogen, Waltham, MA, USA) in a 25 μL reaction according to the manufacturer’s procedure.

    Article Title: LINC00467 Is Upregulated by DNA Copy Number Amplification and Hypomethylation and Shows ceRNA Potential in Lung Adenocarcinoma
    Article Snippet: After 48 h of 5-azacytidine treatment, the total RNA in the A549 cells was isolated using Trizol reagent (Thermo Fisher Scientific). cDNA synthesis was conducted using a SuperScript II RT-PCR kit (Life Technologies), while quantitative PCR was performed using the SYBR Green Master Mix (Life Technologies) with the QuantStudio 3 Real-Time PCR System (Life Technologies) according to the manufacturer’s instructions.

    Article Title: Brief inhalation of sevoflurane can reduce glial scar formation after hypoxic-ischemic brain injury in neonatal rats
    Article Snippet: Complementary DNA was synthesized using an advanced Superscript II RT-PCR kit (Invitrogen).

    Real-time Polymerase Chain Reaction:


    Article Title: EZH2 serves as a viable therapeutic target for myeloma-induced osteolytic bone destruction.
    Article Snippet: Aquantity of 1 μgof the total RNAwas subsequently utilized for reverse transcription (RT) employing the SuperScript II RT-PCR kit (Invitrogen).

    Article Title: LINC00467 Is Upregulated by DNA Copy Number Amplification and Hypomethylation and Shows ceRNA Potential in Lung Adenocarcinoma.
    Article Snippet: After 48 h of 5-azacytidine treatment, the total RNA in the A549 cells was isolated using Trizol reagent (Thermo Fisher Scientific). cDNA synthesis was conducted using a SuperScript II RT-PCR kit (Life Technologies), while quantitative PCR was performed using the SYBR Green Master Mix (Life Technologies) with the QuantStudio 3 Real-Time PCR System (Life Technologies) according to the manufacturer’s instructions.

    Article Title: Mks6 mutations reveal tissue- and cell type-specific roles for the cilia transition zone
    Article Snippet: Quantitative RT-PCR RNA was isolated from Mks6 wild type ( WT ) and Mks6 GT MEFs with Trizol reagent, according to the manufacturer’s protocol (15596-026; Thermo Fisher Scientific, Waltham, MA, USA). cDNA was generated using Superscript II RT-PCR kit (18064; Thermo Fisher Scientific).

    Article Title: EZH2 serves as a viable therapeutic target for myeloma-induced osteolytic bone destruction
    Article Snippet: A quantity of 1 μg of the total RNA was subsequently utilized for reverse transcription (RT) employing the SuperScript II RT-PCR kit (Invitrogen).

    Article Title: TGFBR1 Variants Can Associate with Non-Syndromic Congenital Heart Disease without Aortopathy
    Article Snippet: First-strand cDNA was synthesized from this isolated total RNA (1 μg for both the + RT and -RT reaction) using oligo(dT) primer and the Superscript II RT-PCR kit (Invitrogen, Waltham, MA, USA) in a 25 μL reaction according to the manufacturer’s procedure.

    Article Title: LINC00467 Is Upregulated by DNA Copy Number Amplification and Hypomethylation and Shows ceRNA Potential in Lung Adenocarcinoma
    Article Snippet: After 48 h of 5-azacytidine treatment, the total RNA in the A549 cells was isolated using Trizol reagent (Thermo Fisher Scientific). cDNA synthesis was conducted using a SuperScript II RT-PCR kit (Life Technologies), while quantitative PCR was performed using the SYBR Green Master Mix (Life Technologies) with the QuantStudio 3 Real-Time PCR System (Life Technologies) according to the manufacturer’s instructions.

    Article Title: Brief inhalation of sevoflurane can reduce glial scar formation after hypoxic-ischemic brain injury in neonatal rats
    Article Snippet: Complementary DNA was synthesized using an advanced Superscript II RT-PCR kit (Invitrogen).

    SYBR Green Assay:


    Article Title: EZH2 serves as a viable therapeutic target for myeloma-induced osteolytic bone destruction.
    Article Snippet: Aquantity of 1 μgof the total RNAwas subsequently utilized for reverse transcription (RT) employing the SuperScript II RT-PCR kit (Invitrogen).

    Article Title: LINC00467 Is Upregulated by DNA Copy Number Amplification and Hypomethylation and Shows ceRNA Potential in Lung Adenocarcinoma.
    Article Snippet: After 48 h of 5-azacytidine treatment, the total RNA in the A549 cells was isolated using Trizol reagent (Thermo Fisher Scientific). cDNA synthesis was conducted using a SuperScript II RT-PCR kit (Life Technologies), while quantitative PCR was performed using the SYBR Green Master Mix (Life Technologies) with the QuantStudio 3 Real-Time PCR System (Life Technologies) according to the manufacturer’s instructions.

    Article Title: Mks6 mutations reveal tissue- and cell type-specific roles for the cilia transition zone
    Article Snippet: Quantitative RT-PCR RNA was isolated from Mks6 wild type ( WT ) and Mks6 GT MEFs with Trizol reagent, according to the manufacturer’s protocol (15596-026; Thermo Fisher Scientific, Waltham, MA, USA). cDNA was generated using Superscript II RT-PCR kit (18064; Thermo Fisher Scientific).

    Article Title: EZH2 serves as a viable therapeutic target for myeloma-induced osteolytic bone destruction
    Article Snippet: A quantity of 1 μg of the total RNA was subsequently utilized for reverse transcription (RT) employing the SuperScript II RT-PCR kit (Invitrogen).

    Article Title: TGFBR1 Variants Can Associate with Non-Syndromic Congenital Heart Disease without Aortopathy
    Article Snippet: First-strand cDNA was synthesized from this isolated total RNA (1 μg for both the + RT and -RT reaction) using oligo(dT) primer and the Superscript II RT-PCR kit (Invitrogen, Waltham, MA, USA) in a 25 μL reaction according to the manufacturer’s procedure.

    Article Title: LINC00467 Is Upregulated by DNA Copy Number Amplification and Hypomethylation and Shows ceRNA Potential in Lung Adenocarcinoma
    Article Snippet: After 48 h of 5-azacytidine treatment, the total RNA in the A549 cells was isolated using Trizol reagent (Thermo Fisher Scientific). cDNA synthesis was conducted using a SuperScript II RT-PCR kit (Life Technologies), while quantitative PCR was performed using the SYBR Green Master Mix (Life Technologies) with the QuantStudio 3 Real-Time PCR System (Life Technologies) according to the manufacturer’s instructions.

    Article Title: Brief inhalation of sevoflurane can reduce glial scar formation after hypoxic-ischemic brain injury in neonatal rats
    Article Snippet: Complementary DNA was synthesized using an advanced Superscript II RT-PCR kit (Invitrogen).

    Quantitative RT-PCR:


    Article Title: EZH2 serves as a viable therapeutic target for myeloma-induced osteolytic bone destruction.
    Article Snippet: Aquantity of 1 μgof the total RNAwas subsequently utilized for reverse transcription (RT) employing the SuperScript II RT-PCR kit (Invitrogen).

    Article Title: LINC00467 Is Upregulated by DNA Copy Number Amplification and Hypomethylation and Shows ceRNA Potential in Lung Adenocarcinoma.
    Article Snippet: After 48 h of 5-azacytidine treatment, the total RNA in the A549 cells was isolated using Trizol reagent (Thermo Fisher Scientific). cDNA synthesis was conducted using a SuperScript II RT-PCR kit (Life Technologies), while quantitative PCR was performed using the SYBR Green Master Mix (Life Technologies) with the QuantStudio 3 Real-Time PCR System (Life Technologies) according to the manufacturer’s instructions.

    Article Title: Mks6 mutations reveal tissue- and cell type-specific roles for the cilia transition zone
    Article Snippet: Quantitative RT-PCR RNA was isolated from Mks6 wild type ( WT ) and Mks6 GT MEFs with Trizol reagent, according to the manufacturer’s protocol (15596-026; Thermo Fisher Scientific, Waltham, MA, USA). cDNA was generated using Superscript II RT-PCR kit (18064; Thermo Fisher Scientific).

    Article Title: EZH2 serves as a viable therapeutic target for myeloma-induced osteolytic bone destruction
    Article Snippet: A quantity of 1 μg of the total RNA was subsequently utilized for reverse transcription (RT) employing the SuperScript II RT-PCR kit (Invitrogen).

    Article Title: TGFBR1 Variants Can Associate with Non-Syndromic Congenital Heart Disease without Aortopathy
    Article Snippet: First-strand cDNA was synthesized from this isolated total RNA (1 μg for both the + RT and -RT reaction) using oligo(dT) primer and the Superscript II RT-PCR kit (Invitrogen, Waltham, MA, USA) in a 25 μL reaction according to the manufacturer’s procedure.

    Article Title: LINC00467 Is Upregulated by DNA Copy Number Amplification and Hypomethylation and Shows ceRNA Potential in Lung Adenocarcinoma
    Article Snippet: After 48 h of 5-azacytidine treatment, the total RNA in the A549 cells was isolated using Trizol reagent (Thermo Fisher Scientific). cDNA synthesis was conducted using a SuperScript II RT-PCR kit (Life Technologies), while quantitative PCR was performed using the SYBR Green Master Mix (Life Technologies) with the QuantStudio 3 Real-Time PCR System (Life Technologies) according to the manufacturer’s instructions.

    Article Title: Brief inhalation of sevoflurane can reduce glial scar formation after hypoxic-ischemic brain injury in neonatal rats
    Article Snippet: Complementary DNA was synthesized using an advanced Superscript II RT-PCR kit (Invitrogen).

    Generated:


    Article Title: EZH2 serves as a viable therapeutic target for myeloma-induced osteolytic bone destruction.
    Article Snippet: Aquantity of 1 μgof the total RNAwas subsequently utilized for reverse transcription (RT) employing the SuperScript II RT-PCR kit (Invitrogen).

    Article Title: LINC00467 Is Upregulated by DNA Copy Number Amplification and Hypomethylation and Shows ceRNA Potential in Lung Adenocarcinoma.
    Article Snippet: After 48 h of 5-azacytidine treatment, the total RNA in the A549 cells was isolated using Trizol reagent (Thermo Fisher Scientific). cDNA synthesis was conducted using a SuperScript II RT-PCR kit (Life Technologies), while quantitative PCR was performed using the SYBR Green Master Mix (Life Technologies) with the QuantStudio 3 Real-Time PCR System (Life Technologies) according to the manufacturer’s instructions.

    Article Title: Mks6 mutations reveal tissue- and cell type-specific roles for the cilia transition zone
    Article Snippet: Quantitative RT-PCR RNA was isolated from Mks6 wild type ( WT ) and Mks6 GT MEFs with Trizol reagent, according to the manufacturer’s protocol (15596-026; Thermo Fisher Scientific, Waltham, MA, USA). cDNA was generated using Superscript II RT-PCR kit (18064; Thermo Fisher Scientific).

    Article Title: EZH2 serves as a viable therapeutic target for myeloma-induced osteolytic bone destruction
    Article Snippet: A quantity of 1 μg of the total RNA was subsequently utilized for reverse transcription (RT) employing the SuperScript II RT-PCR kit (Invitrogen).

    Article Title: TGFBR1 Variants Can Associate with Non-Syndromic Congenital Heart Disease without Aortopathy
    Article Snippet: First-strand cDNA was synthesized from this isolated total RNA (1 μg for both the + RT and -RT reaction) using oligo(dT) primer and the Superscript II RT-PCR kit (Invitrogen, Waltham, MA, USA) in a 25 μL reaction according to the manufacturer’s procedure.

    Article Title: LINC00467 Is Upregulated by DNA Copy Number Amplification and Hypomethylation and Shows ceRNA Potential in Lung Adenocarcinoma
    Article Snippet: After 48 h of 5-azacytidine treatment, the total RNA in the A549 cells was isolated using Trizol reagent (Thermo Fisher Scientific). cDNA synthesis was conducted using a SuperScript II RT-PCR kit (Life Technologies), while quantitative PCR was performed using the SYBR Green Master Mix (Life Technologies) with the QuantStudio 3 Real-Time PCR System (Life Technologies) according to the manufacturer’s instructions.

    Article Title: Brief inhalation of sevoflurane can reduce glial scar formation after hypoxic-ischemic brain injury in neonatal rats
    Article Snippet: Complementary DNA was synthesized using an advanced Superscript II RT-PCR kit (Invitrogen).



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